High resolution melting (HRM) analysis for genetic changes in BRCA1/2 gene
نویسندگان
چکیده
منابع مشابه
High resolution melting analysis for gene scanning.
High resolution melting is a new method of genotyping and variant scanning that can be seamlessly appended to PCR amplification. Limitations of genotyping by amplicon melting can be addressed by unlabeled probe or snapback primer analysis, all performed without labeled probes. High resolution melting can also be used to scan for rare sequence variants in large genes with multiple exons and is t...
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Background: The High Resolution Melting (HRM) method is a new scanning method for detecting unknown changes in DNA and its advantages have persuaded researchers to recruit it as a screening method. Objectives: Here, we developed a HRM method to screen R188H SNP (rs3218536) of XRCC2 and compared the results with a well known PCR-RFLP technique. Materials and Methods: Genomic ...
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Hereby we present evaluation of high-resolution melting for mutation scanning applied to the cystic fibrosis transmembrane conductance regulator gene. High resolution melting was used for mutation scanning of selected samples derived from cystic fibrosis patients with a known cystic fibrosis transmembrane conductance regulator genotype. We tested 19 different disease-causing cystic fibrosis tra...
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Running title: Mutations within the RGS genes in breast cancer. Author manuscripts have been peer reviewed and accepted for publication but have not yet been edited. Author manuscripts have been peer reviewed and accepted for publication but have not yet been edited. Abstract Background: Identification of specific mutation targets in cancer may lead to discovery of
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BACKGROUND High resolution melting (HRM) is an emerging new method for interrogating and characterizing DNA samples. An important aspect of this technology is data analysis. Traditional HRM curves can be difficult to interpret and the method has been criticized for lack of statistical interrogation and arbitrary interpretation of results. METHODS Here we report the basic principles and first ...
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ژورنال
عنوان ژورنال: Journal of thee Medical Sciences (Berkala Ilmu Kedokteran)
سال: 2016
ISSN: 0126-1312,2356-3931
DOI: 10.19106/jmedsciesup004804201623